dpph assay for antioxidant activity principle meaning

Oxidative stress plays a significant role in diverse diseases such as cardiovascular conditions, cancer, inflammatory diseases and early ageing (progeria) Citation2, Citation3. Insufficient levels of endogenous and exogenous antioxidants can cause oxidative stress, an imbalance of oxidants and antioxidants resulting in cellular damage or death. Antioxidants protect biological systems against free radical damage. The free radicals formed can cause structural and functional changes in biomolecules. Free radicals are also produced as a consequence of the incomplete reduction of oxygen molecule Citation1. Oxidation is a chemical reaction that transfers hydrogen or electrons from a substance to an oxidising agent, and oxidation reactions can generate free radicals. The advantages of the new method are the use of lower amounts of reagents and solvents, no need for costly spectrophotometers, and thus significantly lowered costs, and convenient implementation in any environment and situation. The spot images were evaluated with Image J software to determine CSC 50 values, the sample concentrations providing 50% colour reduction, which were very similar with the SC 50 values obtained with spectrophotometric method. and standard antioxidants or extracts of common medicinal herbs were dropped onto TLC plates, after an incubation period.In this new method, the mixtures of solutions of DPPH To overcome this drawback, the colorimetric determination of the antioxidant activity using a scanner and freely available Image J software was developed. ) radical scavenging, the most commonly used antioxidant method with more than seventeen thousand articles cited, is very practical however, as with most assays, it has the major disadvantage of dependence on a spectrophotometer.

Dpph assay for antioxidant activity principle meaning